Xenbase Image ID: 79175

Fig. 1. Hormone-dependent induction of notochord in animal cap tissues by co-expression of Xbra-GR and Pintallavis-GR. (A) Schematic illustration of hormone-inducible Xbra and Pintallavis. The hormone-binding domain of the human glucocorticoid receptor (GR) was fused to the carboxy-terminus of full length Xbra and Pintallavis. Stop codons of both genes were replaced with two amino acids (D and L) creating a BglII site (AGATCT) (see Tada et al., 1997). (B) RNAase protection assay of Bix1, a direct target of Xbra, and Xnot in animal caps expressing either Xbra-GR or Pintallavis-GR alone or a combination of the two. Pintallavis-GR alone could not induce either Bix1 or Xnot. Bix1 and Xnot were induced by Xbra-GR alone in a hormone (DEX)-dependent manner. Bix1 expression was induced by co-expression of Xbra-GR and Pintallavis-GR but at a slightly reduced level, while Xnot expression was enhanced. (C) DEX-dependent notochord formation in animal caps co-expressing Xbra-GR and Pintallavis-GR. Animal caps were fixed at stage 41 and stained with the notochord-specific antibody MZ15. Neither Xbra-GR nor Pintallavis-GR alone could induce ectopic notochord formation in animal caps (data not shown). Image published in: Saka Y et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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