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Figure 3 Novel retinal stem cell markers retrieved from the screen. A: Stage 39 retinal sections showing the specific expression in retinal stem cells of the 18 clones isolated from the screen (gene symbol is indicated on each panel). A'': Magnification of the corresponding dorsal CMZ (except for sulf2 in (B0) for which the ventral CMZ is shown). White doted lines delineate the staining. S: Quantification of the staining extent in the dorsal CMZ. Values represent the stained surface relative to total CMZ area and are given as mean +/- SEM (n = at least 6 retinal sections per clone). Note that although all clones are expressed in the stem cell containing region of the CMZ, staining expansion differs from one to another (light grey: less than 25% of the CMZ area; medium grey: between 25 and 45%; dark grey: more than 45%). In addition, major dorso-ventral variability is observed for sulf2, which was thus quantified in both the dorsal and ventral CMZ as indicated. Scale bar = 50 um. Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.
This material is reproduced with permission of John Wiley & Sons, Inc.
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