Xenbase Image ID: 79199

Figure 3. Smad10 Is Required for Expression of Anterior and Posterior Neural Markers and for Formation of Melanocytes(A) Embryos were injected and analyzed by in situ hybridization as in Figure 2B.(B) Albino embryos (albino mother, pigmented father) were examined for the appearance of melanocytes (MC; dark cells visible in the C MO embryo).(C) Embryos were injected and analyzed at early neurula by in situ hybridization as in Figure 2B, except that 12.5 ng of morpholino was injected per cell.(D) Animal caps were explanted at stage 8.5, prior to organizer signaling, and Sox-2 expression was analyzed by RT-PCR.(E) Two-cell embryos were injected with morpholinos and, at the 32-cell stage, β-galactosidase mRNA was injected into the B1 blastomere. At stage 27, the embryos were stained with X-gal (blue). Image published in: LeSueur JA et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 32 optic vesicle forebrain midbrain en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 28 midbrain-hindbrain boundary hoxc9-like.L X. laevis Throughout NF stage 32 neural tube myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 15 presumptive paraxial mesoderm sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 15 neuroectoderm Image source: Published Larger Image Printer Friendly View

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