Xenbase Image ID: 79201

Figure 5. Smad10 Is Required for FGF-Mediated Neural Induction(A) One-cell embryos were uninjected or injected with Smad10 or control MO and then animal caps were treated with FGF protein at the gastrula stage. These caps, and uninjected/untreated caps (Control), were analyzed at stage 27 by whole-mount in situ hybridization.(B) β-gal-injected, β-gal-injected/FGF-treated (FGF + β-gal), and DNS10-injected/FGF-treated animal caps were analyzed by whole-mount in situ hybridization.(C) Animal caps treated as in (A) and (B) were analyzed by RT-PCR.(D) Uninjected, dominant-negative FGF receptor-injected (XFD, 1 ng), and DNS10-injected animal caps were cultured during the blastula stage in the absence or presence of FGF. These caps were then analyzed by RT-PCR at stage 11. Image published in: LeSueur JA et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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