Xenbase Image ID: 79336

Figure 2. Tet3 Directly Regulates Key Developmental Genes. (A) Expression level changes of developmental genes resulting from Tet3 depletion as shown by in situ hybridization at stage 14. Red arrows show the inhibited expression by xlTet3 MOs injection, whereas black arrows show rescued expression by xtTet3 coinjection. L, left; R, right; a, anterior; p, posterior; d, dorsal; v, ventral. The red dots are injection tracer by β-gal staining.(B) RT-qPCR confirms the differentially expressed genes after Tet3 depletion at stage 14. Control MO, xlTet3 MOs or xlTet3 MOs/xtTet3 was injected into two dorsal blastomeres at 4-cell stage. Relative gene expression was normalized to odc. Results are shown as mean ± SD (n = 3).(C) Tet3 occupancy at target gene promoters by ChIP-qPCR assay. Data are presented as mean ± SEM (n = 3).See also Figure S2. Image published in: Xu Y et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 14 pre-chordal neural plate optic field anterior neural fold rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 14 optic field six3.L hpe2, six3-a, six3-b, XSix3, xsix3.1, xsix3.2 X. laevis Throughout NF stage 14 optic field sox9.S cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Throughout NF stage 14 neural crest snai1.S LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 14 neural crest neurog2.L atoh4, neurog2-a, neurog2-b, neurogenin 2, neurogenin-2, neurogenin-related-1, neurogenin2, ngn-2, ngn2, ngnr, ngnr 1a, Ngnr-1, Ngnr1, X-ngnr-1, xngn2, Xngnr-1, XNgnr1 X. laevis Throughout NF stage 14 neuron trigeminal placode tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 14 neural fold shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 14 neural tube Image source: Published Larger Image Printer Friendly View

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