Xenbase Image ID: 79339

Figure 5. The Tet3 CXXC Domain Specifically Recognizes Cytosine through a Conserved Residue His90. (A and C) Crystal structures of the Tet3 CXXC domain in complex with ACGT DNA (5′-GCCAACGTTGGC-3′) (A) or CmCGG DNA (5′-GCCACmCGGTGGC-3′) (C) in cartoon (left) and electrostatic representations (right), respectively. The double-stranded DNA sequence is shown in the middle of each corresponding panel.(B and D) Detailed interactions between the Tet3 CXXC domain and ACGT DNA (B) or between the Tet3 CXXC domain and CmCGG DNA (D). Red arrow, salt bridge interaction; olive arrow, hydrogen bond interaction; yellow arrow, electrostatic interaction.(E and F) Detailed interactions between the Tet3 CXXC domain (blue cartoon) and the target CG pair (E) or the following GC pair (F) in the ACGT DNA (red cartoon).(G and H) Detailed interactions between the Tet3 CXXC domain (blue cartoon) and the target CG pair (G) or the following mCG pairs (H) in the CmCGG DNA (green cartoon).See also Figures S5 and S6. Image published in: Xu Y et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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