XB-IMG-79340
Xenbase Image ID: 79340
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Figure 6. Both 5mC Hydroxylase Activity and the CXXC Domain Are Important for Tet3 Function. (A) Schematic representation of xtTet3 mutants.(B) The CXXC domain deletion disrupts Tet3 occupancy at specific gene promoters by ChIP-qPCR assay. Data are presented as mean ± SEM (n = 3). ∗p < 0.05.(C) Summarized results of five independent pax6 expression rescue experiments. “Suppressed” means significantly suppressed pax6 expression in posterior and anterior neural plates; “Partially suppressed” means pax6 expression is detected but not intact in posterior and anterior neural plates; “Normal” means intact pax6 expression in posterior and anterior neural plates. ∗∗p < 0.01 compared to xlTet3 MOs, # p < 0.01 compared to xlTet3 MOs/xtTet3.(D) Summarized results of five independent phenotypic rescue experiments. “Partial defect” means mild abnormal head structure, small eyes or one eye; “Complete defect” means abnormal head structure and no eye. ∗∗p < 0.01 compared to xlTet3 MOs, # p < 0.01 compared to xlTet3 MOs/xtTet3.(E) A model of Tet3 action in gene transcription regulation. The Tet3 CXXC domain specifically binds to unmodified cytosine (underlined)-containing sequence motifs with a slight preference for G at “Y” position and a mild disfavor for T or 5mC at “X” position, targeting Tet3 to the promoter of target developmental genes. Then, the 5mC hydroxylase activity of Tet3 converts adjacent 5mC to 5hmC, an intermediate for further DNA demethylation, thus activates the gene expression. CD: catalytic domain. Please refer to the related text for more details.See also Figure S7. Image published in: Xu Y et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |