XB-IMG-79350
Xenbase Image ID: 79350
|
Fig. 8. Distinct populations of cells respond to T3 by proliferating (as evidenced by BrdU incorporation — green) or by upregulating the TrβA promoter (as evidenced by EGFP expression — red). Premetamorphic tadpoles (NF stage 52) were transfected with a X. laevis TrβA promoter-EGFP reporter plasmid by electroporation-mediated gene transfer, treated with T3 (50 nM) for 48 h, and then immersed in BrdU for 3 h before sacrifice and harvest of brains for dual labeling fluorescent immunohistochemistry for EGFP and BrdU. Transverse sections through the lateral ventricles were prepared and analyzed by confocal microscopy. 1. A majority of EGFP-positive cells (red — with white arrows) were BrdU negative. The yellow arrow shows one cell with colocalization of EGFP and BrdU. Scale bar = 100 μm. 2. High magnification image showing that most EGFP-positive cells are located at the periphery of the subventricular zone (SVZ), with few in the ventricular zone (VZ). Scale bar = 50 μm. 3. Some EGFP expressing cells exhibited long processes that extended out to the pial surface characteristic of radial glia. Scale bar = 200 μm. 4. Most radial glial cells expressing EGFP were BrdU positive (yellow arrows) with cell bodies located in the SVZ. Scale bar = 50 μm. Image published in: Denver RJ et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |