Xenbase Image ID: 79440

Fig3. 32-cell sizzled morphant phenotypes and rescue. Sizzled morpholino was injected, together with GFP RNA, into the C2/C3 blastomeres of 32 cell stage embryos. Embryos were sorted based on fluorescence at stage 10. Stage 11 embryos were stained for xbra RNA ((A)-control, (B,C)-morphant); stage 18 embryos were stained for snail2 ((D)-control, (E,F)-morphant), twist1 ((G)-control, (H,I)-morphant), sox9 ((J)-control, (K,L)-morphant) RNAs. Effects on myoD RNA were examined at stage 25 ((M)-control, (N)-morphant). (O) sox9 expression in sizzled MO embryos was rescued by sizzled RNA injection (200 pgs/embryo) but not by sizzled D92N RNA (green bars indicate percentage of embryos with a wild type phenotype, red bars indicate loss of sox9 RNA staining). Image published in: Shi J et al. (2012) © 2012. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 marginal zone involuting marginal zone involuted dorsal mesoderm involuted ventral mesoderm snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 18 neural crest cranial neural crest neural plate border chordal neural plate border twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 18 neural crest cranial neural crest sox9.S cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Throughout NF stage 18 neural crest cranial neural crest myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 25 ventral head region myotome Image source: Published Larger Image Printer Friendly View

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