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Fig 4. Sizzled-dependent induction in ectodermal-DLMZ explants. Ectodermal explants were isolated from stage 8/9 embryos and cultured either alone (A) or together with DLMZ explants, isolated from stage 10 wild type (B) or sizzled C2/C3 morphant (C) embryos. When control embryos reached stage 18, explants were stained in situ for sox9 RNA. In similar studies (D), explants were analyzed when control embryos reached stage 11 by qPCR for levels of sox9, snail2, or twist1 RNAs. Levels of these RNAs in whole embryos (WE), animal caps (AC), control animal cap-dorsal mesoderm (AC-DM) or control animal cap-sizzled morphant dorsal mesoderm (sizzled MO) were compared. Image published in: Shi J et al. (2012) © 2012. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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