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Fig 5. Sizzled-dependent changes in DLMZ gene expression and antagonist rescue studies. (A) The DLMZ region (at stage 11) was isolated from C2/C3 sizzled morphant embryos and subjected to qPCR analysis. Compared to control DLMZs (green), sizzled morphant DLMZs (red) showed small but reproducible increases in wnt8 and bmp4 RNA levels, and reproducible decreases in noggin and and sfrp2 RNA levels. (B) We then examined the ability of injection of noggin and sfrp2 RNAs, either alone or together, to rescue the sizzled C2/C3 morphant sox9 phenotype: neither was effective alone, but in combination they produced a robust rescue. Green bars indicated rescued embryos, red bars indicate loss of sox9 expression. (C) Given that SFRP2 may have both anti-Wnt and anti-BMP activities, we examined the behavior of the ureWnt antagonist Dkk. As before, neither noggin or dkk RNAs alone rescued, but together they produced a greater than 50% rescue of sox9 expression. Image published in: Shi J et al. (2012) © 2012. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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