XB-IMG-79445
Xenbase Image ID: 79445
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Fig 2. Cx26 and Cx32 are co-expressed in the epithelial lining of the gastrocoel next to the GRP.
Temporal and spatial expression patterns were determined by WMISH of staged embryos. (A) Cx26. (A) In the early gastrula embryo Cx26 expression around the blastopore is enhanced on the dorsal side (arrowhead). (A′) Histological section shows Cx26 staining in the involuting endodermal cells. (B,C) In the stage 17 neurula embryo Cx26 mRNA is found in the endoderm lining the gastrocoel. (B) Dorsal explant. Outline of GRP, which is free of staining, indicated by dashed line. (C) Sectioning (C′) of whole-mount embryo (C) shows Cx26 expression all around the gastrocoel with the marked exception of the GRP, demonstrated in blowup shown in (C′). (D) Cx26 expression at stage 35. Transversal section (D′) shows staining in the pronephros, the liver diverticulum and in the dorsal half of the endoderm. (E,F) Cx32. (E) Expression in posterior half of stage 18 neurula embryo. Section in (E′) demonstrates staining in two open ring-like domains representing the limit of the endoderm, compassing the endodermal yolk cells. Blowup in (E′) shows that the GRP is free of Cx32 expression. (F) Cx32 expression at stage 30. Transversal hemisection in (F′) reveals mRNA staining in the pronephros, hatching gland and throughout the endoderm. a, anterior; bc, blastocoel; bp, blastopore; d, dorsal; eym, endodermal yolk mass; gc, gastrocoel; l, left; ld, liver diverticulum; no, notochord; nt, neural tube; p, posterior; pn, pronephros; r, right;s, somite; st., stage; v, ventral. The position of the GRP is indicated by dashed lines in (B) and (E). Red arrowheads mark the position of the blastopore in (A,A′). Lines in (C,D,F) indicate planes of histological sections in (C′,D′,F′). Image published in: Beyer T et al. (2012) © 2012. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |