XB-IMG-79446
Xenbase Image ID: 79446
Fig 3. Cx26 and Cx32 form heteromeric connexons in Xenopus embryos.
Fusion constructs (Cx26-eGFP and Cx32-RFP) were co-injected into the animal region of single blastomeres at the 4-8 cell stage and cultured through blastula-gastrula stages. Fluorescent fusion proteins were co-expressed in ectodermal cells of the animal cap region. Expression was found in vesicles (white arrowheads) and the plasma membrane (blue arrowheads). (A) Cell boundaries. Positions highlighted in (B) are indicated by arrowheads. (B) Cx26-eGFP staining. (C) Cx32-RFP staining. (D) Overlay of frames. Scale bar represents 10 . Image published in: Beyer T et al. (2012) © 2012. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |