Xenbase Image ID: 79493

Figure 1. The manual mark-up annotation tool in use. The annotator is presented with the in situ image to annotate and the appropriate schematic diagram (which they may override). The task is to identify the regions of in situ stain on the image and transfer them as faithfully as possible to the schematic, making mental allowance for any rotation, inversion or more complex distortion required. In situ images are shown on the top row, annotations on the schematic diagram are shown on the bottom row; red represents strong staining, orange is weak staining. On the left, an image of a gastrula stage 10 embryo is shown, viewed from the vegetal pole with the blastopore side up. In the middle, a stage 15 neurula is shown viewed from the anterior region, dorsal side up. On the right is shown a stage 22 early tailbud, viewed from the side; dorsal is to the top, but in this case the embryo has been photographed unconventionally with anterior to the right, and the annotator has had to flip the annotation 180° on a vertical axis. Schematic diagrams: copyright 1994 from “Normal Table of Xenopus laevis” by Nieuwkoop and Faber. Reproduced by permission of Garland Science/Taylor & Francis, LLC. Image published in: Gilchrist MJ et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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