XB-IMG-79500
Xenbase Image ID: 79500
|
Fig. 3. Phenotypic effects of δNXMyD88 mRNA on axis formation: embryos were injected dorsally into both blastomeres at two-cell stage, based on pigmentation differences between the prospective dorsal and ventral sides (see Section 4.2 for details). (A) Series of typical axis truncations induced by injection of δNXMyD88 mRNA (50 pg). From top to bottom: no effect (DAI 5), reduced forehead (DAI 4), cyclopia (DAI 3), microcephalic and headless (DAI 2). (B) Phenotypic spread of embryos: black, uninjected control embryos (n=234); red, embryos injected with 50 pg (n=162) or blue, injected with 1000 pg (n=210) δNXMyD88 RNA; green, rescue experiment with embryos coinjected with 50 pg δNXMyD88 RNA and 100 pg XMyD88 (n=117). (C) Phenotypic penetrance as a function of injection timing. X-axis indicates the developmental stage, at which δNXMyD88 RNA was injected. (D,E) δNXMyD88-injected embryos lacked notochord (MZ15 staining): (D) Control embryo; (E) δNXMyD88-injected embryo (DAI 2). (F–H) Sagittal sections through δNXMyD88-injected embryos at the level of the otic vesicle (ot, otic vesicle; nt, neural tube; not, notochord, so, somites): (F) Control embryo; (G) microcephalic embryo without notochord, impaired neural tube and somite structure; (H) cyclopic embryo with slightly aberrant somites. N≥3 independent experiments. Image published in: Prothmann C et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |