Xenbase Image ID: 79507

Figure 5. xYAP gain-of-function inhibits the expression of genes in the pre-placodal ectoderm, epidermis, premigratory neural crest, and hatching gland.(A) Genes expressed in the pre-placodal ectoderm (PPE), sox11 and six1, are dramatically reduced on the xYAP-injected sides (arrows). Brackets indicate the laterally located PPE expression domains on both sides the embryos. Anterior views. (B) Expression of the epidermis-specific cyto-keratin gene is lost on the xYAP-injected side. Anterior view. (C) The expression of genes characteristic of premigratory neural crest (foxD3, zic1 at bracket) are repressed on the xYAP-injected sides. Anterior-dorsal views. (D) pax3 expression in the surface ectodermal A-P stripe, which indicates the hatching gland progenitors (vertical arrows, HG) is repressed on the xYAP-injected side. In contrast, pax3 expression in the underlying neural crest progenitors is expanded (see Figure 6A). Dorsal view. (E) six1 expression in the otocyst (bracket) is reduced on the xYAP-injected side (right panel) compared to the uninjected side (Un) of the same embryo (left panel). Side views, stage 26. (F) The migratory path of foxD3-expressing neural crest cells (indicated by brackets) is truncated on the xYAP-injected side (right). Frontal view, stage 24. (G) neuroD expression in the trigeminal placode (bracket) is reduced on the xYAP-injected side (right panel) compared to the uninjected side (Un) of the same embryo (left panel). Side views, stage 22. Image published in: Gee ST et al. (2011) Image downloaded from an Open Access article in PubMed Central. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Gene Synonyms Species Stage(s) Tissue sox11.L sox11-a, sox11-b, XLS13, xls13a, XLS13b, xSOX-11, xsox11 X. laevis Throughout NF stage 18 neural plate preplacodal ectoderm six1.L XSix1 X. laevis Throughout NF stage 18 preplacodal ectoderm krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 18 epidermis non-neural ectoderm foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 18 cranial neural crest zic1.S odd-paired-like, opl, Zic-r1, Zic-related-1 X. laevis Throughout NF stage 18 cranial neural crest neural plate border pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 18 ectoderm hatching gland cranial neural crest six1.L XSix1 X. laevis Throughout NF stage 24 otic placode olfactory bulb cephalic nerve cranial nerve foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 24 hyoid crest cranial neural crest mandibular crest anterior branchial crest posterior branchial crest neurod1.L neurod, neuroD, neurod1-a, neurod1-b, XNeuroD X. laevis Throughout NF stage 22 trigeminal placode Image source: Published Larger Image Printer Friendly View

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