Xenbase Image ID: 79509

Figure 7. Endogenous xYAP resides at a novel 5′ regulatory region of pax3.(A) A highly conserved putative TEAD-binding site (yellow boxes) is present in the 5′ regulatory region of the pax3 gene in 15 different vertebrates. A previously described mouse TEAD-binding site (red box) appears less conserved. (B) Chromatin isolated from 300 wild type stage 14–16 Xenopus laevis embryos was sheared to a size range of 150 to 900 base pairs. (C) Chromatin immunoprecipitations (ChIPs) from 12.5 µg or 25 µg of sheared chromatin immunoprecipitated a band at the expected size for the putative novel TEAD1-binding site region with the hYAP antibody but not with a control IgG antibody. (D) Sequencing of this band from three different clones verified that the genomic region pulled down by the hYAP anitbody contained the novel TEAD-binding site (yellow) when compared to the Xenopus tropicalis genomic sequence. Image published in: Gee ST et al. (2011) Image downloaded from an Open Access article in PubMed Central. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Larger Image Printer Friendly View

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