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Figure 3. Suppresor of cytokine signaling-3 (SOCS3) expression is rapidly induced after epithelial wounding. A: Embryos were subjected to superficial epithelial wounds, allowed to heal for the stated times (0–8 hr) and then subjected to in situ hybridization using a SOCS3 antisense riboprobe. SOCS3 is first evident at 30 min after trauma and is maximal at 1 hr. The wound-induced expression of SOCS3 is transient and dramatically down-regulated 2 hr after trauma and is undetectable by 8 hr after trauma. B: SOCS3 is rapidly induced after trauma at early stages of embryonic development. Embryos at the gastrula stage (stage 11) were wounded and allowed to heal for 1 hr before fixation and in situ hybridization for SOCS3 expression. C: SOCS3 expression is induced in the epidermis and underlying tissues after trauma. Cross-section (8 μm) of a stage 28 embryo after wounding, healing for 1 hr, and then analyzed for SOCS3 expression by in situ hybridization. SOCS3 expression (blue staining) in evident in the epidermis and the underlying tissues. Arrow indicates the site of wounding, and the parallel dotted lines indicate the depth of the epidermal layer. Original magnification, ×200. N, notochord; S, somite; fg, foregut; e, epidermis. Download figure to PowerPoint Image published in: Kuliyev E et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue socs3.L atod4, cis3, cish3, socs-3, ssi-3, ssi3, xsocs3 X. laevis Throughout NF stage 11 wound epidermis socs3.L atod4, cis3, cish3, socs-3, ssi-3, ssi3, xsocs3 X. laevis Throughout NF stage 28 wound epidermis wound epidermis inner layer wound epidermis outer layer Image source: Published Larger Image Printer Friendly View

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