Xenbase Image ID: 79700

Fig. 1. Loss of H3.3/H3 leads to gastrulation arrest in Xenopus embryos. (A) Western blot analysis of cell lysates (H3 and β-actin) or acid-extracted nuclear fractions (H3.3 and H3) from stage 11 control or H3 MO-injected embryos. (B) Time-lapse images tracking the development of a representative pair of control and H3 MOs-injected embryos from early to late gastrulation. (C) Protein synthesis of injected mRNAs shown by western blot analysis of cellular extracts from embryos injected with H3 MOs and 750 pg of mRNA encoding either HA-epitope-tagged mouse H3.3 or H2Az. (D) Morphology of injected embryos and control siblings at stage 12.5. (E) Percentage of injected embryos arrested at early (stage 10.5-11) or late (stage 11.5-12) gastrula stages. Image published in: Lim CY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page