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XB-IMG-79701

Xenbase Image ID: 79701

Fig. 2. H3.3/H3-depleted Xenopus embryos fail to express mesodermal marker genes. (A-I′) Control and injected embryos fixed at early gastrula were subjected to RNA in situ hybridization for the analysis of mesodermal (A-C′), endodermal (D-F′) and ectodermal (G-I′) marker gene expression. Representative embryos from three experiments are shown. (A-D,G) Vegetal views; (E,F) lateral views of bisected embryos, dorsal towards the right; (H) lateral view; (I) animal view. (J) Expression of selected genes in control and H3 MO-injected embryos at stage 10.5 was measured by qRT-PCR. All values were normalized to ornithine decarboxylase (ODC) and plotted relative to the respective transcript levels in control embryos. Error bars indicate s.d. of three independent experiments. *P<0.05 using a two-tailed Student t-test.

Image published in: Lim CY et al. (2013)

Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
tbxt.SXbra, X-bra, bra, Xbrachyury, brachyury, ntl, t-a, t-b, tX.laevisThroughout NF stage 10mesoderm
dorsal marginal zone
gsc.LXgsc, goosecoid, gsc-a, gsc-bX.laevisThroughout NF stage 10mesoderm
dorsal marginal zone
myf5.LXmyf5, Xmyf-5, myf-5X.laevisThroughout NF stage 10mesoderm
marginal zone
dorso-lateral marginal zone
sox17a.LxSox17alpha1, xSox17alpha2, tSox17alpha, Xsox17-alpha, Sox17alpha, sox17-alpha, xsox17a, xsox17, Xsox17alpha, sox17 alphaX.laevisThroughout NF stage 10endoderm
vegetal endoderm
blastopore
hhex.LXHex, tHex, hexX.laevisThroughout NF stage 10endoderm
endomesoderm
cer1.Sxcer, xcer-1, xcer1, cer, Cerberus, tCerberus, cer-1, dand4X.laevisThroughout NF stage 10endomesoderm
endoderm
chrd.1.Schd, chordin, X-chordin, chrd, loc108716939X.laevisThroughout NF stage 10dorsal marginal zone
ectoderm
krt12.4.Lepidermal keratin, epi-keratin, epiker, cytokeratin, Epidermis-specific keratin, xK81, EpK, cytok, E-keratin, ck81, K81, EpiK, xk81a1, xk81aX.laevisThroughout NF stage 10ectoderm
epidermis
animal hemisphere
foxi1.LXema, ectodermally-expressed mesendoderm antagon, FoxI1e, xfoxi1, ema, foxi1e, foxi3X.laevisThroughout NF stage 10epidermis
animal hemisphere
ectoderm

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