Xenbase Image ID: 79701

Fig. 2. H3.3/H3-depleted Xenopus embryos fail to express mesodermal marker genes. (A-I′) Control and injected embryos fixed at early gastrula were subjected to RNA in situ hybridization for the analysis of mesodermal (A-C′), endodermal (D-F′) and ectodermal (G-I′) marker gene expression. Representative embryos from three experiments are shown. (A-D,G) Vegetal views; (E,F) lateral views of bisected embryos, dorsal towards the right; (H) lateral view; (I) animal view. (J) Expression of selected genes in control and H3 MO-injected embryos at stage 10.5 was measured by qRT-PCR. All values were normalized to ornithine decarboxylase (ODC) and plotted relative to the respective transcript levels in control embryos. Error bars indicate s.d. of three independent experiments. *P<0.05 using a two-tailed Student t-test. Image published in: Lim CY et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10 mesoderm dorsal marginal zone gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10 mesoderm dorsal marginal zone myf5.L myf-5, Xmyf-5, Xmyf5 X. laevis Throughout NF stage 10 mesoderm marginal zone dorso-lateral marginal zone sox17a.L sox17 alpha, sox17-alpha, Sox17alpha, tSox17alpha, xsox17, xsox17a, Xsox17-alpha, Xsox17alpha, xSox17alpha1, xSox17alpha2 X. laevis Throughout NF stage 10 endoderm vegetal endoderm blastopore hhex.L hex, tHex, XHex X. laevis Throughout NF stage 10 endoderm endomesoderm cer1.S cer, cer-1, Cerberus, dand4, LOC108713989, tCerberus, xcer, xcer-1, xcer1 X. laevis Throughout NF stage 10 endomesoderm endoderm chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10 dorsal marginal zone ectoderm krt12.4.L ck81, cytok, cytokeratin, E-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, epi-keratin, epK, EpK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 10 ectoderm epidermis animal hemisphere foxi1.L ectodermally-expressed mesendoderm antagonist, ema, FoxI1e, foxi1e, foxi3, Xema, xfoxi1 X. laevis Throughout NF stage 10 epidermis animal hemisphere ectoderm Image source: Published Larger Image Printer Friendly View

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