XB-IMG-79704
Xenbase Image ID: 79704
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Fig. 5. Mesodermal competence is regulated by interplay of nucleosomal H3 and linker histone H1. (A) Nucleosomal ladders resulting from micrococcal nuclease digests of nuclei from control, H3.3 H1Aand H3.3+H1Aknockdown embryos. Arrows point to di-, tri- and tetra- nucleosomes; M is the 123bp DNA ladder. (B) Oligomeric DNA length was calculated and plotted against the nucleosome number. Error bars indicate s.d. of six independent experiments. The mean nucleosomal repeat length (NRL) in each set of embryos is indicated with s.d. (C) Embryos injected with H3 MOs alone, H1A MO alone or H3 and H1A MOs were either cultured to late gastrula stage 12.5 (top panels), or fixed at stage 10.5 and subjected to in situ hybridization analysis of Xbra expression (bottom panels). (D) Animal caps excised from stage 8 control or embryos injected with H1A (1.5 ng), H3.1 (1.5 ng), H3.3 (1 ng) or combinations of H1A+H3.1/H3.3 mRNAs, were exposed to Activin A at late blastula and early gastrula stages, and cultured until stage 25. Image published in: Lim CY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |