XB-IMG-79782
Xenbase Image ID: 79782
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Fig. S2. Overexpression of H3.3 leads to early embryonic lethality. (A) Embryos at the two- to four-cell stages were injected with 1 or 2 ng of mRNA encoding HA-tagged H3.1 or H3.3. Injected embryos were either allowed to develop to late tailbud stage, or fixed at stage 10.5 and subjected to whole-mount in situ hybridization analysis of Xbra expression. (B) Percentage of injected embryos that developed normally to late gastrula stage 12.5. (C) Synthesis of exogenous H3.1- HA or H3.3-HA proteins in injected embryos detected by immunoblotting for the HA epitope. Image published in: Lim CY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |
