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Fig. S4. Presence of RNA polymerase II and H3.3 at the promoters of active genes in gastrula embryos. (A) Presence of endogenous RNA polymerase II at the promoters of Xbra, myf5 and cebpa were analyzed by ChIP-qPCR analysis of stage 10.5 control and H3 MOs-injected embryos. Level of enrichment is determined as a percentage of input. (B) Chromatin immunoprecipitation using anti-HA antibody was performed on stage 10.5 control and embryos injected with 750 pg of mH3.3a-HA mRNA. Presence of H3.3-HA at the promoters of Xbra, siamois and cebpa were analyzed by PCR using promoter-specific primers. Image published in: Lim CY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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