Xenbase Image ID: 79785

Fig. S5. Mesoderm formation and gene expression in H3 MOs morphants is rescued by co-injection of mouse H3f3a mRNA. (A) Control and injected embryos fixed at early gastrula were subjected to RNA in situ hybridization analysis of Xbra and gsc transcripts. (B) Percentage of embryos co-injected with mRNA encoding HA-tagged wild-type or mutant H3.3, or H3.1 that arrested at early (stage 10.5-11) or late (stage 11.5-12) gastrula stages. (C) Rescue experiments were performed by co-injecting H3.3 MO with a total amount of 750 pg of mRNA encoding HA-tagged H3.1 or H3.3, or both. Injected embryos were either allowed to develop to late gastrula stage 12.5 (top panels), or fixed at stage 10.5 and subjected to whole-mount in situ hybridization analysis of Xbra expression (bottom panels). (D) Percentage of injected embryos that arrested at early (stage 10.5-11) or late (stage 11.5-12) gastrula stages. Image published in: Lim CY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10 mesoderm marginal zone gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10 mesoderm dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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