Xenbase Image ID: 79839

Fig. 1. Activation/repression of Xwnt-8 expression as a function of BMP-4 RNA dose. The effect of changes in the level of Xwnt-8 expression as a function of the level of BMP-4 in LiCl-dorsalized embryos. The expression level of Xwnt-8 was analyzed by RT-PCR and the phenotype of LiCl-treated embryos was determined at stage 34/35. (A) RT-PCR analysis of Xwnt-8 expression in LiCl-dorsalized embryos injected with increasing amounts of BMP-4 mRNA, histone H4 served as a loading control. (B) Control untreated embryos. (C) Phenotype of embryos dorsalized by partial LiCl treatment. (D) Embryos dorsalized by partial LiCl treatment and injected with 0.1 ng of BMP-4 mRNA appear almost normal. (E) Injection of 0.5 ng of BMP-4 capped RNA into embryos treated with partial LiCl results in reversal of the LiCl effect to a ventral phenotype. (F) Further increase in the amount of BMP-4 mRNA injected to 1.0 ng into partial LiCl dorsalized embryos results in strong ventralization. Image published in: Marom K et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page