Xenbase Image ID: 79840

Fig. 2. Repression of Xwnt-8 by high levels of BMP-4. Embryos were injected dorsally with increasing amounts of BMP-4 mRNA. At stage 10.5 the dorsal part of the marginal zone was cut including the dorsolateral region and expression of Xwnt-8 in this region was studied by RT-PCR. Sibling embryos were allowed to develop to stage 40/41 for phenotypic analysis of the injected RNAs. (A) Schematic description of the experiment. (B) RT-PCR analysis of Xwnt-8 expression in embryos injected with 0.2 or 1 ng of BMP-4 mRNA, histone H4 served as a loading control. (C) Control untreated embryo. (D) Embryo injected with 0.2 ng of BMP-4 RNA exhibiting intermediate ventralization. (E) Injection of 1 ng of BMP-4 mRNA resulted in strong ventralization. Image published in: Marom K et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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