XB-IMG-79844
Xenbase Image ID: 79844
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Fig. 6. Regulation of myf-5 by Xwnt-8 and BMP-4 during late gastrulation. Embryos were injected with mRNAs to manipulate the Xwnt-8 and the BMP-4 signaling pathways and the effects of these manipulations on myf-5 expression were studied. All embryos were co-injected with LacZ RNA to mark the site of injection (red). Following injection, the embryos were dorsalized by LiCl treatment. Injected embryos were fixed at stage 12 and studied by in situ hybridization (blue). (A) Control embryo exhibiting the normal myf-5 pattern of expression. (B) LiCl dorsalization suppresses the expression of myf-5. (C) High levels of BMP-4 overexpression can activate myf-5 expression but the activation is in regions bordering the site of injection. (D) Co-injection of BMP-4 and dominant negative Xwnt-8 encoding mRNAs show that the activation of myf-5 by BMP-4 requires active Xwnt-8 signaling. (E) Injection of 60 ng of an Xwnt-8 expression plasmid resulted in the activation of myf-5 expression at the site of injection. (F) Higher amounts of injected Xwnt-8 expression plasmid (160ng) result in the repression of myf-5 at the site of injection but expression can be observed in neighboring regions. (G,H) Co-injection of the Xwnt-8 expression plasmid together with antisense BMP-4 RNA did not change the effect on myf-5 expression. The red arrowhead marks the site of injection and the black arrowhead marks the in situ hybridization signal. Image published in: Marom K et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V.
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