Xenbase Image ID: 79869

FIG. 4. XCdc42 overexpression affects the localization of mesodermal and neural markers. Four-cell stage embryos were injected in the DMZ with 200 pg of WT XCdc42 mRNA. (A) (a) Expression of chordin at stage 10 in uninjected control (upper) and XCdc42-injected (lower) embryos (vegetal view is shown; dorsal at top). (b) Expression of chordin in uninjected (upper, dorsovegetal view; anterior at top) and XCdc42-injected (lower, vegetal view; dorsal at top) embryos at stage 12. In XCdc42-injected embryos, chordin-expressing cells did not converge toward the dorsal midline and were still localized around the blastopore. (c) The pattern of Xbra expression in uninjected (upper) and XCdc42-injected (lower) embryos at stage 10 (vegetal view; dorsal at top). (d) Expression of Xbra in stage 12 control (upper, dorsovegetal view) and XCdc42-injected (lower, vegetal view; dorsal at top) embryos. Xbra transcripts, which were detectable in the notochordal mesoderm in uninjected control embryos, were absent in XCdc42-injected embryos, indicating the failure of dorsal mesoderm to involute during gastrulation. (e, f) The expression of XmyoD in uninjected (dorsovegetal view) and XCdc42-injected (vegetal view; dorsal at top) embryos at stage 11.5, respectively. In XCdc42-injected embryos, XmyoD remained expressed around the blastopore, but not in the lateral mesoderm. (g) At stage 13, uninjected control embryos had the anterior expression of Otx-2 (dorsal view is shown; anterior at top). (h) In XCdc42-injected embryos at stage 13, the expression of Otx-2 occurred in the dorsal ectoderm (dorsovegetal). (B) (a, b) In situ hybridization against XmyoD was performed on both uninjected (a) and XCdc42-injected (b) embryos at stage 25. XCdc42-injected embryos failed to close the neural plate, yet retained muscle differentiation. (c, d) As in the uninjected embryo at stage 30 (c), notochord differentiation normally occurred in the XCdc42-injected embryo (d) as assessed by expression of Xnot. Image published in: Choi SC and Han JK (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10 dorsal marginal zone tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10 marginal zone myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 11.5 presumptive paraxial mesoderm otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 13 pre-chordal neural plate optic field not.S flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot X. laevis Throughout NF stage 29 and 30 head brain pharyngeal arch eye cement gland notochord tail tip chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 12 axial mesoderm tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 12 mesoderm marginal zone ventral marginal zone ventro-lateral marginal zone dorso-lateral marginal zone dorsal marginal zone involuting marginal zone involuted dorsal mesoderm involuted ventral mesoderm myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 25 presomitic mesoderm Image source: Published Larger Image Printer Friendly View

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