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XB-IMG-79985

Xenbase Image ID: 79985


Figure S4. Interaction-Domain Analyses of p53 and XFDL (A) Schematic of the deletion mutants of human p53 used in (B). Venus/GFP was fused at the amino-terminus of each construct as a tag for immunoprecipitation. (B) Immunoprecipitation analysis of the deletion mutants of hp53 and XFDL. HEK293 cells were transfected with indicated expression plasmids. Immunoprecipitation was performed with the anti-GFP antibody and the protein was detected with the anti-FLAG antibody (top row). Expression of each construct was confirmed by Western blot (middle row for FLAG-fused XFDL and bottom row for Venus-fused proteins). (C) Schematic of the deletion mutants of XFDL used in (D) and (E). FL; FLAG tag. (D) Immunoprecipitation analysis of the deletion mutants of XFDL and human p53 (hp53). HEK293 cells were transfected with indicated expression plasmids. Immunoprecipitation was performed with the anti-HA antibody and the proteins were detected with the anti-FLAG antibodies (top row). Expression of each construct was confirmed by Western blot (middle row for FLAG-fused XFDL and bottom row for HA-fused p53). (E) Immunoprecipitation analysis of the deletion mutants of XFDL and hp53. Immunoprecipitation was performed with the anti-HA antibody and the proteins were detected with the anti-GFP antibody (top row). Middle row, Venus-fused XFDL; bottom row, HA-fused hp53. Arrows; coprecipitated Venus-fused proteins.

Image published in: Sasai N et al. (2008)

Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.

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