Xenbase Image ID: 79986
Figure S8. Expression of Fast1 and Fast3 Is Regulated by XFDL (A-H) Expression profiles of Fast1 (A-D) and Fast3 (E-H) in vivo. Embryos at the 8-cell stage (A, E), stage 8.5 (B, F), stage 11 (C, G) and stage 12 (D, H) were analyzed by in situ hybridization with Fast1 (A-D) and Fast3 (E-H) probes. An; animal, Veg; vegetal, A; anterior, P; posterior. D; dorsal, V; ventral. Arrowheads, rim of the blastopore. (I-O) Expression of Fast1, Fast3 and p53 in the embryos injected with XFDL mRNA. Control embryos (I, K, M) and embryos injected with XFDL (400 pg/cell; J, L, N) at the 4-cell stage were analyzed at stage 11 by in situ hybridization with the Fast1 (I, J), Fast3 (K, L) and p53 (M, N) probes. (O) Expression of Fast1, Fast3 and p53 in the animal caps. XFDL mRNA (400 pg/cell; lane 2 in each panel) was injected at the 8-cell stage and animal caps were prepared at stage 8.5. Explants were cultured until stage 11 and expression of Fast1 (left graph), Fast3 (middle graph) or p53 (right graph) was analyzed by q-PCR. Expression level of each gene in the control animal cap (lane 1) was defined as 100%.
Image published in: Sasai N et al. (2008)
Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
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