Xenbase Image ID: 79993

Fig. 6. Expression analyses of CREBA133PR injected embryos. Representative embryos are shown that were injected with RNA encoding CREBA133PR, treated with RU486 at stage 12, and used for whole mount in situ hybridization analyses at stage 30 to visualize the expression pattern of MyoD, Otx2 and Shh. Note that in CREBA133PR-injected and RU486-treated embryos the fore- and midbrain expression domains of Otx2 are not well separated from each other and that the MyoD expression domain in the somites is split and does not show the typical V-shaped form of normal somites. The expression of Shh has not changed. Injected embryos without RU486 treatment developed normally and did not show any changes in expression of MyoD, Otx2 and Shh. Scale bar: 500 mm; scale bar for insets: 100 mm. Image published in: Lutz B et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 28 somite epaxial muscle otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 28 eye forebrain midbrain shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 28 central nervous system brain spinal cord pharyngeal arch head region Image source: Published Larger Image Printer Friendly View

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