Xenbase Image ID: 79996

Figure 3. Downregulation of non-transcription factor candidate target genes after knockdown of EBF2 and EBF3. One cell of two-cell stage embryos was injected with either control MO, both EBF2 MO and EBF3 MO (2MO + 3MO), or dominant negative Xenopus EBF3 (DN-XEBF) mRNA. β-Galactosidase (β-gal) mRNA was co-injected as a marker of the injected side. In all panels the right side is the injected side, showing the light blue color of X-gal staining. (A-R) The expression of pcdh8 (B,C), peripherin (E,F), greb1 (H,I), nf-m (K,L), kcnk5 (N,O), and activin beta b (Q,R) is downregulated by EBF2 MO and EBF3 MO, and by DN-XEBF (brackets), while control MO does not change their expression levels (A,D,G,J,M,P). Panels (A-I) and (P-R) are neurula stage embryos (stage 15/16), and (J-O) are tail bud stage embryos (stages 25 to 28). All panels show dorsal views. Image published in: Green YS and Vetter ML (2011) Copyright ©2011 Green and Vetter; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue prph.L nef4, peripherin, XIF3 X. laevis Throughout NF stage 15 neuron Rohon-Beard neuron trigeminal placode chordal neural plate border inhbb.L activin B, activin beta b, inhibin, XactBB1, XactBB2, XactBetaB1, XactBetaB2 X. laevis Throughout NF stage 15 to NF stage 16 pre-chordal neural plate presumptive midbrain-hindbrain boundary anterior neural fold optic field pre-chordal neural plate border greb1.L X. laevis Throughout NF stage 15 midbrain-hindbrain boundary neural plate chordal neural plate anterior neural ridge non-neural ectoderm kcnk5.S k2p5.1, task-2, task2 X. laevis Sometime during NF stage 25 to NF stage 28 hindbrain spinal cord eye midbrain-hindbrain boundary otic placode pcdh8.L papc, paraxial protocadherin, xpapc X. laevis Throughout NF stage 15 neuron chordal neural plate border nefm.L nef-m, nef3, nefm-a, nefm-b, nf-m X. laevis Sometime during NF stage 25 to NF stage 28 spinal cord neuron trigeminal ganglion Image source: Published Larger Image Printer Friendly View

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