Xenbase Image ID: 80033
Fig. 4. Mutations induced by TALENs are heritable through the germline.
(A) F1 cleavage stage embryos produced by mating F0 frogs previously injected with mRNAs encoding TALENs targeting the tyr gene, give rise to a mixture of albino and pigmented embryos. (B) F1 tadpoles produced by mating F0 frogs previously injected with mRNAs encoding TALENs targeting the tyr gene, give rise to a mixture of albino tadpoles, flanking a pigmented sibling tadpoles (centre). (C) The tyr loci were amplified by PCR from three randomly selected F1 embryos (F1-1 to 3), and PCR clones from these embryos were sequenced. One was homozygous wild type (top), the other two were heterozygous mutant. F1-a represents sequence from an albino F1 embryo, which confirmed that the embryo was homozygous mutant. Genotype and frequency of sequence are shown in the right of the each allele. Scale bar: 1 mm.
Image published in: Ishibashi S et al. (2012)
Image downloaded from an Open Access article in PubMed Central. © 2012. Published by The Company of Biologists Ltd
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