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Fig. 4. Effect of lin28 knockdown on FGF and activin/nodal signalling. (A,C) Animal cap explants from control and lin28 morphant X. tropicalis embryos, with or without FGF (A) or activin (C) treatment. (B,D) Histology on sections of animal caps from A and C, respectively. ae, atypical epidermis; bl, blood; mes, mesenchyme; ms, muscle. (E) Western blots showing dpERK expression levels in animal cap explants from control and lin28 morphant embryos, with or without activin or FGF treatment. GAPDH, loading control. (F) Expression levels of FGF4 and FGF8 in lin28 morphants at stage 10.5 relative to controls as assessed by Q-PCR. Solid line represents mRNA levels in corresponding control embryos (set at 1). **P<0.005 (Student t-test), for morphant expression compared with control expression. Error bars indicate s.d. Image published in: Faas L et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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