XB-IMG-80040
Xenbase Image ID: 80040
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Fig. 7.
let-7 in Xenopus development. (A) Q-PCR analysis of let-7a, let-7f and let-7g expression from stage 9 to stage 22. Expression is calibrated to stage 22 for each gene. (B-D) RNA EMSA supershifts showing let-7g terminal loop RNA-binding activity of dilutions of protein extracted from Xenopus embryos overexpressing lin28a1 (B), lin28a2 (C) or lin28b (D) proteins. Lane 1, labelled let-7g terminal loop alone; lanes 2 and 3, let-7g plus 1:4 and 1:8 dilutions of control embryo lysate, respectively; lanes 4 and 5, let-7g plus 1:4 and 1:8 dilutions, respectively, of lysate from lin28-overexpressing embryos; lane 6, let-7g plus 1:4 dilution of control embryo plus relevant lin28 antibody; lane 7, let-7g plus 1:4 dilution of control embryo lysate plus relevant pre-immune serum; lane 8, let-7g plus 1:4 dilution of lin28-overexpressing embryo lysate plus relevant lin28 antibody; lanes 9, let-7g plus 1:4 dilution of lin28-overexpressing embryo lysate plus relevant pre-immune serum. (E) Q-PCR analysis of relative expression of let-7 miRNAs in lin28 morphants compared with control embryos at stage 10.5 and 22. Solid line represents miRNA levels in corresponding control embryos (set at 1). *P<0.05 (Student t-test), for morphant compared with control expression. Error bars indicate s.d. Image published in: Faas L et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |