Xenbase Image ID: 80102

Fig. 3. Syn4 overexpression neuralises ectoderm. (A-D) Anterior view, dorsal to the top (A,B); ventral view (C,D). (A,C) 500 pg of NLS-β-gal mRNA was injected into the A4 blastomere of a 32-cell stage Xenopus embryo, and the expression of Sox2 (A) and epidermal keratin (Epk) (C) was analysed by in situ hybridisation. (B,D) 1 ng of Syn4 mRNA was injected into the A4 blastomere of a 32-cell stage embryo, and the expression of Sox2 (C; 96% of induction, n=87) and Epk (D; 80% of inhibition, n=70) was analysed. (E,F) Higher magnification of embryos injected as in B,D. Arrows indicate nuclei stained with X-Gal (blue). (G-J) Dorsal (G,I) and ventral (H,J) views of embryos injected as in B with Syn4 mRNA. No ectopic expression of MyoD (0%) or Xbra (0%) was observed. Inset in H shows injected fluorescein. (K-N) Animal cap (AC) assay for Sox2 (K,L) or Epk (M,N) expression. (K,M) Control caps express only Epk. (L,N) Syn4 mRNA-injected animal caps express Sox2 and downregulate Epk. Image published in: Kuriyama S and Mayor R (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 14 neural plate neuroectoderm krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 14 ectoderm non-neural ectoderm myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 14 presumptive paraxial mesoderm tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 14 circumblastoporal collar axial mesoderm Image source: Published Larger Image Printer Friendly View

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