Xenbase Image ID: 80103

Fig. 4. The FGF/MAPK pathway is required for neural induction by Syn4. (A-F) Sox2 expression analysed by whole-mount in situ hybridisation in Xenopus animal caps. (A) Control caps. No Sox2 expression. (B) Syn4-injected caps express Sox2. (C) Co-injection of Syn4 and a dominant-negative form of FGF receptor 1 (XFD-1) inhibits Sox2 expression. (D) Syn4-injected caps treated with 40μ M SU5402 (in DMSO) do not show Sox2 expression. (E) Syn4-injected caps treated with 80 μM U0126 do not express Sox2. (F) Syn4-injected caps treated with 80 μM U0124 show expression of Sox2.(G) Animal caps were injected as indicated (Cont, control) and samples taken for western blot analysis of MAPK phosphorylation at the equivalent of stage 12. Antibodies against MAPK or phosphorylated MAPK (p-MAPK) can recognise both p42 and p44 as a single band. Each experiment was repeated three times with at least 50 animal caps. Image published in: Kuriyama S and Mayor R (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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