XB-IMG-80106
Xenbase Image ID: 80106
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Fig. S1. Syn4/PKC/Rac pathways involved in neural induction. Embryos were injected as indicated into the animal blastomeres of an 8-cell stage embryo or into A1 or A4 of a 32-cell embryo, and the expression of neural or epidermal markers analyzed at stage 19. (A) Control MO injected at the 8-cell stage. Analysis of Nrp1 (0% inhibition, n=18). (B) Syn4 MO injected at the 8-cell stage. Analysis of Nrp1 (96% inhibition, n=27). (C) PKCδ mRNA injected at the 8-cell stage. Analysis of Nrp1 (46% inhibition, n=23). (D) Constitutively active Rac mRNA injected at the 8-cell stage. Analysis of Nrp1 (39% inhibition, n=42). (E) PKCδ mRNA injected at the 32-cell stage. Analysis of NCAM (43% inhibition, n=7). (F) Constitutively active Rac mRNA injected at the 32-cell stage. Analysis of NCAM (32% inhibition, n=25). (G) Sox2 expression of embryo injected with PKCα and a dominant-negative form of FGF receptor (XFD-1). Note that the ectopic induction is not inhibited (58% induction, n=31). (H) Epidermal keratin (Epk) expression after injection of PKCα mRNA into A4 blastomeres. Note the inhibition of epidermis (63% inhibition, n=22). Image published in: Kuriyama S and Mayor R (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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