Xenbase Image ID: 80107

Fig. S2. Syn4 has a neuralising activity that does not depend on BMP inhibition. (A,B) Formation of secondary axis. (A) Embryos were injected in ventral blastomeres with Syn4 (A) or chordin (B) mRNA and the lineage tracer β-galactosidase. Note the secondary axis induced by chordin injection; but no secondary axis was observed in Syn4-injected embryos. (C,D) Sox3 expression in stage 14 embryos, dorsal to the top. Inset shows the dorsal side. (C) Control expression of Sox3. (D) Embryo was injected with Syn4 mRNA into A4 blastomeres and the expression of Sox3 analyzed. Note the ectopic induction of Sox3 in ventral epidermis (89% induction, n=49). (E-G) Neuralisation by Syn4 does not require chordin. (E) Ventral view of an embryo injected with Syn4 mRNA. Note the ectopic Sox2 expression (91%, n=12). (F) Ventral view of embryo co-injected with Syn4 mRNA and chordin MO. There was no effect on the ectopic induction of Sox2 (89%, n=37). (G) Embryo injected with chordin MO to show the expected ventralised phenotype (100%, n=9). (H,I) Sox2 expression analysed in stage 20 embryos. Anterior view, dorsal to the top. (H) Control. (I) Embryo injected in A4 with Syn4 mRNA. Note the strong ectopic induction of Sox2. (J,K) Animal caps showing induction of Otx2 by Syn4 expression (K); no induction was observed in control animal caps (J). Image published in: Kuriyama S and Mayor R (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 20 neural plate ectoderm neuroectoderm preplacodal ectoderm sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 16 neural plate ectoderm neuroectoderm preplacodal ectoderm Image source: Published Larger Image Printer Friendly View

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