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Figure S2. Smad 2 is an intracellular component of activin/nodal signaling, thus smad2-expressing AC induced chordamesoderm tissue cell autonomously. RT-PCR analysis showed that AC receiving over 250 pg smad2 mRNA expressed the chordamesoderm marker. When the AC was co-cultured with Smad2-AC, EB3 in the AC cells moved randomly, unlike that in the AC cells co-cultured with Activin expressing AC, which moved toward the boundary in a mirror image. These results suggest that the boundary between two distinct tissues has information to attract the EB3 movement; moreover, the attracting cue is specific for chordamesoderm tissue, because the EB3 in the AC moved randomly near the tissue boundary. Image published in: Shindo A et al. (2008) Shindo et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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