XB-IMG-80179
Xenbase Image ID: 80179
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Figure 2. mRNA expression of Xenopus zfp36 genes during development.
(A) RT-PCR analyses showed that all zfp36 genes are maternally expressed. zfp36, zfp36l1 and zfp36l2 mRNAs are expressed at a constant level throughout development from stage 2 to stage 33 while zfp36l4 mRNA level decreases after the mid-blastula transition (MBT, arrow). (B) In situ hybridization showed that all four zfp36 mRNA are localized at the animal pole in 4-cell stage (a) and morula stage (i) embyos. e correspond to histological sections from embryos shown in a. (C) RT-PCR analysis showed that zfp36 mRNAs are preferentially expressed in the animal pole region of blastula embryos. (D) RT-PCR analysis showed that zfp36 mRNAs are expressed throughout the embryo at the gastrula stage. An, animal pole; DM, dorsal marginal zone; Emb, whole embryo; Ve, vegetal pole; VM, ventral marginal zone. A control embryo (Emb) assayed by RT-PCR for the expression of control genes chordin and wnt8. odc was used as control of loading and a reaction was performed in the absence of reverse transcriptase to check for genomic DNA contamination (-). Image published in: Tréguer K et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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