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Figure 4. The different zfp36 genes respond differently to growth factors treatment in animal cap explants. (A) RT-PCR analysis of zfp36 gene expression in animal cap explants treated with 12.5 or 50 ng/ml of FGF2 or with the FGF inhibitor in the absence (SU) or in the presence of 50 ng/ml FGF2 (SU+F). (B) RT-PCR analysis of zfp36 gene expression in animal cap explants treated with 1, 5 or 25 ng/ml of activin or with the activin inhibitor in the absence (SB) or presence of 25 ng/ml activin (SB+A). (C) RT-PCR analysis of zfp36 genes in on animal cap explants from embryos injected with 0.5 ng or 1 ng of BMP2 mRNA. Stage 20 embryo (Emb) or uninjected embryo or untreated animal caps (-) were assayed by RT-PCR for the expression of control genes msr, myl1 and globin. Odc was used as control of loading and a reaction was performed in the absence of reverse transcriptase to check for genomic DNA contamination (-RT). Image published in: Tréguer K et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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