XB-IMG-80183
Xenbase Image ID: 80183
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Figure 6. Zfp36 mRNA overexpression does not prevent mesoderm induction nor myogenic factor expression. (A) Two-cell stage embryos were injected with 250 pg of the different Xenopus zfp36 mRNAs or mouse zfp36 mRNA (mzfp36) and animal caps were explanted at stage 8.5 then treated with 10 ng/ml of activin before analysis by RT-PCR for xbra expression when control embryos reached stage 12. Stage 12 embryo (Emb) or untreated animal caps (-) were assayed by RT-PCR in parallel. Odc was used as control of loading and a reaction was performed in the absence of reverse transcriptase to check for genomic DNA contamination (-RT). (B) 250 pg of Xenopus zfp36 (a, b) and zfp36l1 (c, d) or mouse Zfp36 (mZfp36, e, f) mRNAs were injected in one blastomere of two-cell stage embryos and developing embryos were fixed at stage 28 and analyzed by in situ hybridization for myod expression. Image published in: Tréguer K et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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