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Figure 2. The cell mass is required for the intracellular calcium increase in chordamesodermal cells. (A) Single frames picked from the time-lapse analysis of calcium dynamics in isolated single chordamesodermal cells (G-CaMP (green cytoplasm) and membrane RFP (red)) upon their attachment to ectodermal tissue (below the dotted line, membrane RFP) taken by each 40 seconds. (B) Calcium dynamics of the chordamesodermal tissue (Fluo 4-AM (green cytoplasm) and membrane RFP (red)) when isolated single ectodermal cells (surrounded by a dotted line) were attaching to it. The same experiments were performed using G-CaMP. They did not show the active signals in either (A) or (B). (iso.meso: isolated chordamesoderm, ecto: ectoderm, iso.ecto: isolated ectoderm, meso: chordamesoderm). doi:10.1371/journal.pone.0008897.g002 Image published in: Shindo A et al. (2010) Shindo et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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