XB-IMG-80377
Xenbase Image ID: 80377
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Fig. 1. Spiking cells in the developing neural tube are postmitotic neurons. (A) Ca2+ imaging of the ventral spinal cord of a stage-24 (26-h-postfertilization) embryo for 20 min. Circles identify cells spiking during 20-min recording. Inset shows Ca2+ spike activity for the cell outlined in yellow. (B) (Left) After imaging, the same preparation was whole-mount immunostained for Sox2 and N-β-tubulin. (Right) Immunostaining of a transverse section of the spinal cord from a stage-24 embryo. (C) Ca2+ imaging of an open-book spinal cord preparation. (D) Whole-mount immunostaining of the same preparation for Sox2 and N-β-tubulin. (E) Diagram of the open-book spinal cord preparation shown in C and D. D, dorsal; V, ventral. (Scale bars, 20 μm.) Image published in: Belgacem YH and Borodinsky LN (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |