Xenbase Image ID: 80385

FIG. 4. In vivo suppression of the Wnt-4 overexpression phenotype by Wnt-4 antisense morpholinos. Both blastomeres at the two-cell stage were injected with either 0.25 ng Wnt-4 mRNA alone or Wnt-4 mRNA and Wnt4-MO (0.25 ng each). RNA encoding cytoplasmic 􏰁-galactosidase mRNA (cyt􏰁gal) was coinjected as a lineage tracer. Embryos were fixed at stage 33/34, processed for 􏰁gal activity, and classified according to their phenotype. (A) Injection of Wnt-4 mRNA frequently causes a shortened body-axis phenotype. In the experiment shown, 31% (n 􏰈 25) of the embryos were normal or mildly affected. Moderate phenotypes were observed in 64% (n 􏰈 52) and severe truncations were found with 5% (n 􏰈 4) of the embryos. Examples of embryos with phenotypes ranging from mild to severe are shown. (B) Coinjection of Wnt4-MO rescues Wnt-4-injected embryos. Out of the injected embryos (n 􏰈 41), 85% developed with no discernable phenotype. Examples of rescued embryos are shown. A mild shortened body-axis phenotype was observed with 15% of the embryos. Scale bars: 1.1 mm (A); 1 mm (B) Image published in: Saulnier DM et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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