XB-IMG-80387
Xenbase Image ID: 80387
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Fig. 6. Coinjection of HAWnt-4 rescues SGLT-1L expression in Wnt4-MO-treated Xenopus embryos. Single V2 blastomeres of eight-cell stage embryos were injected with 5 ng Wnt4-MO alone (A) or in conjunction with 0.25 ng HAWnt-4 mRNA (B, C). Cytoplasmic beta-galactosidase mRNA (cytBgal; 0.1 ng) was coinjected as a lineage tracer. Tailbud embryos (stage 33/34) were processed for Beta-gal activity and hybridized with antisense SGLT-1L probes. (A) Injection of 5 ng Wnt4-MO abolishes pronephric expression of SGLT-1L. The enlargement of the pronephric tubule region shows the presence of Beta-gal-expressing cells (pale blue), but absence of SGLT-1L transcripts. (B) Control side of an embryo coinjected with Wnt4-MO and HAWnt-4 mRNA. The inset illustrates normal SGLT-1L expression in the pronephros. (C) Injected side of the embryo shown in (B). The enlargement of the bordered area reveals substantial expression of SGLT-1L in the pronephros. Note that Beta-gal-expressing cells (arrowhead) are adjacent or colocalize with SGLT-1L-expressing cells (arrow). Scale bars: 600 um (A); 120 um (insets). Image published in: Saulnier DM et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |