Xenbase Image ID: 80388

FIG. 7. Disruption of pronephric tubule formation by Wnt4-MO injection. Single V2 blastomeres of eight-cell-stage embryos were injected either with 5 ng Wnt4-MO (A) or Wnt4(mp)-MO (B). cytBgal mRNA (0.1 ng) was coinjected to allow identification of the MO-injected side. Embryos were fixed at stage 33/34, processed for cytBgal activity, and hybridized for SGLT-1L expression. Transverse sections (A, 1.5 um; B, 3 um) of plastic-embedded embryos were cut at the level of the pronephros. Selected sections counterstained with Toluidine Blue and basic fuchsine (A, B) are shown with enlargements of the pronephric tubule region of control (A', B') and injected (A", B") sides. (A', B') Well-developed pronephric tubule epithelia (arrowheads) were present on the control sides. (A") In contrast, no pronephric tubules could be detected when Wnt4-MO was injected. An asterisk indicates the position where tubules would normally form. (B") Injection of Wnt4(mp)-MO did not affect development of pronephric tubules (arrowheads). Scale bars: 250 um (A, B); 75 um (A', A", B', B"). Image published in: Saulnier DM et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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