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Figure 2 Overexpression of Xfz3 promotes ectopic expression of genes regulating eye development. A whole-mount in situ hybridization analysis was made of stage 14 embryos (a) that were either uninjected (a, c, and e) or Xfz3 injected (b, d, and f) and then labeled for Xpax6 (a and b), Xrx (c and d), or Xotx2 expression (e and f). Arrows indicate modest expansion of Xrx or Xotx2 expression (d and f). Stage 28 embryos (g) are either uninjected (g, i, and k) or Xfz3 injected (h, j, and l) and then labeled for Xpax6 (g and h), Xrx (i and j), or Xotx2 expression (k and l). Arrows indicate ectopic expression in injected embryos (h, j, and l). β-Galactosidase (sky blue) marks the regions of the embryo derived from the injected blastomere. Image published in: Rasmussen JT et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 14 eye neural plate pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 28 eye rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 14 anterior neural fold pre-chordal neural plate optic field rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 28 eye midbrain pineal gland otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 14 presumptive midbrain-hindbrain boundary neural plate anterior placodal area otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 28 eye brain forebrain midbrain Image source: Published Larger Image Printer Friendly View

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