XB-IMG-80462
Xenbase Image ID: 80462
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Fig. 6. In vivo lipofection of Math5 promotes retinal bipolar cell fates. Embryos were transfected at stage 18 in the region of the optic vesicle with DNA for GFP alone (A) or GFP in combination with Xath5 (B), Math5 (C) or Mash1 (not shown). At stage 41 these embryos were fixed, cryosectioned and stained with Hoechst dye to visualize the retinal cell layers. GFP-labeled cells were scored in retinal sections by laminar position and morphology. In section of embryos transfected with Xath5, there are more labeled cells in the RGC layer (B), while sections of embryos transfected with Math5 show both a larger number of GFP-labeled cells overall and more labeled bipolar cells (C). Bipolar cells were scored as cells with distinct cell bodies in the INL and with thin processes extending radially but not extending into the ONL or GCL. These can be distinguished from Müller glial cells, which are radially oriented but have a more complex morphology (see Dorsky et al, 1995). (D) Percentage of retinal cell types labeled following transfection with DNA for GFP (green, n=432 cells from 5 eyes), GFP plus Xath5 (red, n=939 cells from 5 eyes), Math5 (navy, n=866 cells from 7 eyes) or Mash1 (light blue, n=440 cells from 6 eyes). Xath5 data are derived from Kanekar et al. (1997) and are included here for comparison. Expression of Xath5 biases progenitors toward RGC fate, while both Math5 and Mash1 promote the formation of bipolar cells. The per cent representation of each cell type was calculated as a weighted average. Error bars indicate SEM; asterisk, P<0.01 by Student t-test. Cell types on the graph are listed from left to right in rough order of birth. Abbreviations: G, retinal ganglion cell; B, bipolar cell; P, photoreceptor; A, amacrine cell; H, horizontal cell. Scale bar 0.5 μm. Image published in: Brown NL et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |